|
KMID : 0357319960310010069
|
|
Journal of the Korean Society for Microbiology
1996 Volume.31 No. 1 p.69 ~ p.74
|
|
|
Production and Characterization of Monoclonal Antibodies to Human Nerve Specific Antigen
|
|
|
|
|
Abstract
|
|
|
Peripheral nerve damage is commonly observed in leprosy patients, but the pathogenic processes of nerve damage are still not well understood. In the present study, we produced monoclonal antibodies to protein components from human central nerve
system
(CNS) in an attempt to investigate the immunological mechanisms of nerve damage and to detect nerve antigen from sera in leprosy patients. Seven monoclonal antibodies (six were IgG1 and one was IgG2a in iso type) were produced; five antibodies
were
reactive with 38 kDa AND 43 kDa molecules from human CNS; other two antibodies were cross-reactive with human myelin basic protein and human muscle protein in ELISA. No antibody was reactive with rabbit peripheral nerve system protein. Although
further
evaluations of these antibodies are required to know the specific binding site of CNS protein and the reactivity to other tissue protein, this study suggests that the monoclonal antibodies may be useful in studying the immunological mechanisms of
nerve
damage in leprosy.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|